Chromosome Variation Analysis of Transformed Corneal Cell Lines
- VernacularTitle:传代培养角膜细胞的染色体遗传变异率分析
- Author:
zhi-min, MA
;
yue-e, JIN
;
zhi-hua, REN
;
lou, LU
;
zhen-yu, LU
- Publication Type:Journal Article
- Keywords:
cultured cell lines;
chromosome variation;
corneal cells
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the stability of chromosome variant ratio of three available transformed corneal cell lines. Methods Chromosome specimens of transformed cells including human corneal epithelial cells(HCE),bovine corneal endothelial cells(BCE) and rabbit corneal epithelial cells(RCE) were prepared by a direct method using regular Giemsa staining. Chromosomes of cells in metaphase were counted under the microscope. Then, the variant ratio of chromosomes and their nuclear types were analyzed. Results The chromosome numbers were 56 to 65, 27 to 34 and 74 to 88 for HCE, BCE and RCE, respectively. Chromosome numbers in the three commonly used and transformed corneal cell lines were changed in comparison to their parent tissues. Conclusion Genotyping study may provide important information for using HCE、BCE、RCE in functional studies.
