Inhibitory Effect of Curcumin on H_2O_2-Induced Production of Nitric Oxide and Reactive Oxygen Species in Mouse Embryo-Fibroblast
- VernacularTitle:姜黄素对过氧化氢诱导小鼠巨噬细胞内一氧化氮及活性氧生成的影响
- Author:
yun-qi, GUO
;
li-hong, PEI
;
xin-hui, LIU
- Publication Type:Journal Article
- Keywords:
curcumin;
inducible nitric-oxide synthase;
nitric oxide;
reactive oxygen species;
mouse
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of curcumin on H2O2-induced the production of nitric oxide(NO) and reactive oxygen species(ROS) in mouse embryo-fibroblast.Methods Macrophage were collected in abdominal cavity of 6-8 weeks Kunming mouse,cultured macrophage(2?108 L-1) were divided into control group and curcumin groups randomly.Macrophage in H2O2 group were added into a single bolus of H2O2(1 mmol?L-1) for 30 min,macrophage in curcumin group were preincubated with different concentration of curcumin for 2 h followed by a 30 min incubation with 1 mmol?L-1 H2O2 and macrophage in control group were added into the same volume(0.1 mL) of 9 g?L-1 so-dium chloride.Immunocytochemistry was used to measure the contents of inducible nitric-oxide synthase(iNOS),NO production was determined by Griess reactive,ROS was determined by DCFH-DA Fluorescence proe.Results The production of NO in control group was little.The production of NO in H2O2 group markly highter than that in control group(P
