Significance of Hypoxia Ischemia on Cerebral Cortex Injury and Neurofunctional Development in Immature Rats Brain
- VernacularTitle:缺氧缺血对未成熟大鼠皮质损伤及神经功能的影响
- Author:
li-li, SONG
;
zhi-heng, HUANG
;
chao, CHEN
- Publication Type:Journal Article
- Keywords:
hypoxia-ischemia;
cerebral cortex;
neurofunctional development;
neonatal rat
- From:Journal of Applied Clinical Pediatrics
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish brain hypoxic-ischemic (HI) model using postnatal day 3(P3) SD rats and evaluate the apoptotic neuronal cells in cerebral cortex and neurofunctional development.Methods The P3 rats were randomly divided into HI group (n=35) and control group(n=18).HI was induced in P3 rats with right carotid artery ligation followed by 2.5 h hypoxia in 60 mL?L-1 oxygen at 37 ℃.The injury of neural cells in the cerebral cortex was evaluated by tumor necrosis factor receptor-1(TNF-R1),Caspase-3 immunostaining and Hematoxylin-Eosin (HE) staining in 24 h and 7 d after HI,respectively.Furthermore,the neurofunctional development was evaluated by negative geotaxis reflex and eye opening time.The data were analyzed by SPSS 12.0 software.Results Caspase-3 and TNF-R1 positive cells were abundant in the ipsilateral cortex at 24 h after HI,compared with contralateral part and control group(P
