Effect of Progesterone on Expressions of Superoxide Dismutase and Matrix Metalloproteinase-3 in Brain Tissues of Neonatal Rats with Hypoxic-Ischemic Brain Damage
- VernacularTitle:孕酮对缺氧缺血性脑损伤新生大鼠脑组织超氧化物歧化酶和基质金属蛋白酶3表达的影响
- Author:
shuang, LI
;
chun-yang, XU
;
hong-bo, JIANG
;
dong-liang, LI
- Publication Type:Journal Article
- Keywords:
progesterone;
hypoxic-ischemic brain damage;
blood brain barrier;
matrix metalloproteinase-3;
superoxide dismutase
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of superoxide dismutase(SOD)and matrix metalloproteinase-3(MMP-3) in the brain tissues of newborn rat after hypoxic-ischemic(HI) and the effects of progesterone(PROG) on SOD and MMP-3 expression.Methods Seventy-two neonatal SD rats were randomly divided into 4 groups:normal control group(normal group),sham-operated group,HI group and PROG group.The model of neonatal rat with hypoxic-ischemic brain damage(HIBD) was made.The permeability of the blood-brain barrier was observed by using transmission electron microscopy,the level of SOD in the brain was assessed by hydroxylammoniumchloride autoxidation,MMP-3 protein expression in cerebral cortex part of the brain was detected by immunohistochemical.SPSS 12.0 software was used to analyze the data.Results In normal group and sham-operated group,capillary vessel′s morphology of blood brain barrier of neonatal rats were integrity and smooth,and structure were clarity.The permeability of blood-brain barrier in HI group was obviously enhanced and had slight changes in PROG group.Compared with the sham-operated group,the level of SOD in HI group significantly decreased(P
