Expression of Tumor Necrosis Factor Ligand-Related Molecule-1A in Mice with Viral Myocarditis and Role of Astragaloside
- VernacularTitle:肿瘤坏死因子配体相关分子1A在病毒性心肌炎中的表达及黄芪甲甙的干预作用
- Author:
hong-ying, LIU
;
li-chun, LIU
;
shuang-jie, LI
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor ligand-related molecule-1A;
viral myocarditis;
astragaloside
- From:Journal of Applied Clinical Pediatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of tumor necrosis factor(TNF) ligand-related molecule-1A(TL1A) in mice with viral myocarditis(VM) and the role of astragaloside.Methods Fifty-five male Balb/c mice were randomly divided into 4 groups:control group,model group,low-dose intervention group and high-dose intervention group.Mice in model group,low-dose intervention group and high-dose intervention group were inoculated with 0.1 mL coxsackie B3 virus intraperitoneally.Then,mice in low-dose intervention group and high-dose intervention group were treated with 10 g?L-1 and 90 g?L-1 astragaloside solution,respectively.Mice in control group and model group were treated with 0.1 mL carboxymethycellulose solution.All mice were killed on the 15thday.Histological cross sections of heart were stained with hematoxylin-eosin and myocardial histopathologic scores were counted under optical microscope.The expressions of myocardial TL1A mRNA and protein were detected by reverse transcription polymerase chain reaction and immunohistochemistry.Results The mortality were 0,46.7%,40.0% and 13.3% in control group,model group,low-dose intervention group and high-dose intervention group,respectively.Compared with model group and low-dose intervention group,the mortality was significantly lower in high-dose intervention group(?2=9.46,8.95,Pa
