Expression of Signal Transducers and Activator of Transcription 3 in Airway Epithelial Cells of Asthmatic Mouse Model and Its Role in Airway Remodeling
- VernacularTitle:支气管哮喘小鼠呼吸道上皮细胞信号转导与转录活化因子3的表达及其在呼吸道重塑中的作用
- Author:
yan-ming, LIU
;
guang-min, NONG
;
shu-quan, LI
- Publication Type:Journal Article
- Keywords:
asthma;
airway remodeling;
signal transducers and activators of transcription 3;
epithelial cell
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of signal transducers and activators of transcription 3(STAT3) in airway epithelial cells of asthmatic mouse model and its association with airway remodeling,explore the role of signal-transduction pathway in airway remodeling.Methods Thirty Balb/c mice were randomly divided into normal control group(n=10),asthma without intervention group(n=10) and AG490 intervention group(n=10).The mice were sensitized with ovalbumin to establish the asthmatic model.The histological changes were evaluated by HE staining,total brochial wall thickness(Wat)and smooth muscle thickness(Wam) were measured by image analysis system,the percentages of collagen deposition were detected by Masson′s trichrome staining,the expression of STAT3 in airway were detected by immunohistochemistry technique;lung tissue extracts were analyzed for phosphorylation of STAT3(p-STAT3)by Western blot.SPSS 13.0 software was used to analyze the data.Results 1.The histological changes including airway thickness,airway smooth cell proliferation and excessive collagen deposition in subepithelial aera were found under light microscope in asthmatic mice.2.The level of STAT3 and p-STAT3 in asthma without intervention group were significantly higher than those in normal control group(Pa
