Changes of Type Ⅰ Collagen in Pulmonary Arteries of Rats with Monocrotaline-Induced Pulmonary Hypertension
- VernacularTitle:野百合碱诱导肺动脉高压大鼠肺动脉Ⅰ型胶原的变化
- Author:
chen, LIANG
;
hong-fang, JIN
;
shu-xu, DU
;
jun-bao, DU
- Publication Type:Journal Article
- Keywords:
pulmonary hypertension;
monocrotaline;
collagen;
pulmonary vascular structural remodeling
- From:Journal of Applied Clinical Pediatrics
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of typeⅠcollagen in pulmonary arteries of rats with monocrotaline(MCT)-induced pulmonary hypertension(PH) and explore the mechanism of pulmonary vascular structural remodeling.Methods Twelve male Wistar rats were randomly divided into 2 groups: the MCT group(n=6) and the control group(n=6),which received a single intraperitoneal injection of MCT solution(60 mg?kg-1,the first day) or 9 g?L-1saline,respectively.After 3 weeks,mean of pulmonary artery pressure(mPAP),the value of right ventricle/(left ventricle plus septum)[RV/(LV+S)] and body weight were measured.Lung sections(HE stained) were observed under lightmicroscope for changes of the pulmonary arteries.The protein expression of typeⅠcollagen in pulmonary arteries was detected by immunohistochemical technique.Results Three weeks after MCT injection,compared with control group,mPAP and RV/(LV+S) increased significantly in MCT group[mPAP:(10.60?2.06) mmHg(1 mmHg=0.133 kPa) vs(32.40?3.24) mmHg,P
