Mechanisms of Calcineurin Signaling Pathway Mediating Myocardium Apoptosis of Right Heart in Rats with Chronic Hypoxia
- VernacularTitle:钙调神经磷酸酶信号通路介导大鼠慢性缺氧致右心室心肌凋亡的机制
- Author:
xiao-hong, XU
;
jian-xin, TAN
;
hua-jun, FENG
- Publication Type:Journal Article
- Keywords:
calcineurin;
signaling pathway;
hypoxia;
cardiac apoptosis;
cyclosporine;
rat
- From:Journal of Applied Clinical Pediatrics
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate calcineurin signaling pathway which mediates myocardium apoptosis of right heart in rats with chronic hypoxia and the potential mechanisms of it.Methods A rat model of right ventricular hypertrophy(RVH) was established induced by chronic hypoxia(95-105 mL?L-1 O2).Used randomized block design based on different brood,30 rats were divided into 3 groups:treatment group with cyclosporine A(CsA,10 mg?kg-1?d-1,intraperitoneal injection),chronic hypoxia group,normal control group(with normal oxygen).The rats in CsA treatment group and chronic hypoxia group were exposed to normobaric chronic hypoxia(95-105 mL?L-1 O2,21 days).Apoptotic index(AI),calcineurin A?(CnA?) mRNA levels,Bcl-2 mRNA levels and the protein expression levels of CnA?,nuclear factor 3 of activated T cells(NFAT3) and Bcl-2 in right ventricle were investigated.Results 1.The AI of treatment group with CsA was higher than that in chronic hypoxia group(P
