Effect of Exogenous Hydrogen Sulfide on Expression of Lung Muc5ac and Cytokines in Ovalbumin-Induced Asthma Rats
- VernacularTitle:外源性硫化氢对哮喘大鼠细胞因子及肺Muc5ac表达的影响
- Author:
qiao-bin, CHEN
;
lang, CHEN
;
li-fang, WU
;
li-li, HAN
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
asthma;
muc5ac;
ovalbumin;
Th1/Th2 cytokines;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of gaseous signal molecule hydrogen sulfide(H2S)on the expression of lung Muc5ac and Th1/Th2 cytokines in ovalbumin(OVA)-induced asthma rats and explore the effect of H2S on asthma.Methods Twenty-six healthy SD rats were randomly divided into 3 groups:control group(Group A,n=8),asthma group(Group B,OVA induced,n=9)and NaHS group(Group C,OVA induced rats treated with NaHS,n=9).Twenty-four hours after treatment,rats were anatomized to measure serum interleukin(IL)-4,interferon-? and the levels of infiltration into inflammatory cells around bronchus were observed,which were scored with the optical microscope.The expression of lung Muc5ac was determined by immunohistochemical staining.Results The score of the levels of infiltration into inflammatory cells around bronchus expressed by median was 1 score in group A,4 score in group B and 2 score in group C.There were dramatically statistics significance among the 3 groups(H=13.75 P
