Expression and Regulation of Tight Junction Proteins Occludin in Alveolar Epithelia Cells in Hyperoxia Induced Chronic Lung Injury in Newborn Rats
- VernacularTitle:高体积分数氧致慢性肺疾病新生大鼠肺泡上皮细胞紧密连接蛋白的表达及其调控作用
- Author:
dong-mei, YUE
;
xin-dong, XUE
- Publication Type:Journal Article
- Keywords:
hyperoxia;
epithelia cells;
platelet-derived growth factor;
tight junction proteins
- From:Journal of Applied Clinical Pediatrics
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the change of occludin mRNA in the lung tissue under hyperoxia induced lung injury condition and their regulation of platelet-derived growth factor B(PDGFB).Methods Three hundred and twenty newborn rats were divided into 4 groups accor-ding to different oxygen concentrations(FiO2):experimental group 1(FiO2=800 mL/L),experimental group 2 (FiO2=600 mL/L),experimental group 3(FiO2=400 mL/L) and room-air control group(FiO2=210 mL/L).Rats were killed at 1st,3rd,5th,7th and 14th day respectively during the experiment,the expression of occludin was examined by reverse transcription polymerase chain reaction(RT-PCR) method.The expression of PDGFB in the lung tissue was also observed by immunohistochemistry.SPSS 11.5 software was used to analyze the data.Results The expressions of PDGFB in the lung tissue in experimental group 1 and experimental group 2 were lower than those of the control group at 1th day(Pa
