Effect of AngiotensinⅡ on Myocardial Fibroblasts Proliferation and Their Signal Transduction Mechanism
- VernacularTitle:血管紧张素Ⅱ对新生大鼠心肌成纤维细胞增殖及其信号转导机制的影响
- Author:
shu-qin, CHEN
;
tao, CHEN
;
tai-guang, ZHOU
- Publication Type:Journal Article
- Keywords:
angiotensinⅡ;
myocardial fibroblasts;
protien kinase C;
ventricular reconstitution
- From:Journal of Applied Clinical Pediatrics
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of angiotensin Ⅱ(AngⅡ) on myocardial fibroblasts(MFs) proliferation,the expression and transposition of protein kinase C epsilon(PKC?) and alpha(PKC?),and to find out the mechanism of AngⅡpromoting proliferation and signal trarsduction.Methods The primary culture neonate rat's MFs was used depending on the different time of cell adherence,by the method of immunohistochemical method identifying MFs,2-4 generations MFs were divided into experimental group and control group,experimental group was added with AngⅡ 10-6 mol/L,and nothing was added to control group.Colorimetric method of metrazolium salt(MTT) was used to detect the MFs proliferation; indirect immunofluorescence was used to detect the distribution and location of PKC? and PKC?,then Image-Pro-Plus 4.0 was used to add up fluorescence intensity.Results 1.The number of MFs in experimental group increased much more than that in control group and there was obviously statistical significance(P
