Effect of Erythropoietin on Apoptosis of Human Renal Tubular Cells Induced by Postasphyxial-Serum of Neonates
- VernacularTitle:促红细胞生成素对窒息新生儿血清诱导人近曲肾小管上皮细胞凋亡的影响
- Author:
gang, DENG
;
wen-bin, DONG
;
qing-ping, LI
;
xiao-ping, LEI
;
tao, XIONG
;
cun-liang, DENG
;
feng, CHEN
- Publication Type:Journal Article
- Keywords:
erythropoietin;
asphyxia;
serum;
apoptosis;
Caspase-3;
X-linked inhibitor of apoptosis protein;
infant,newborn
- From:Journal of Applied Clinical Pediatrics
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of erythropoietin(EPO) on apoptosis of human renal tubular(HK-2) cells induced by postasphyxial-serum of neonates.Methods HK-2 cells were used as target cells.The experiment was divided into 4 groups,control group(n=8):HK-2 cells were maintained in standard medium;asphyxia group(n=8):HK-2 cells were treated with serum obtained from neonates with asphyxia.Each culture medium replaced with 200 mL/L suffocation DMEM/F12 serum culture medium;EPO pretreatment group(n=8):HK-2 cells were pretreated 24 h with serum contains 5?104 IU/L EPO,and then deal as asphyxia group;EPO and 5-hydroxydecanoic acid sodium salt(5-HD) pretreatment group(n=8): HK-2 cells were pretreated 24 h with serum contains 5?104 IU/L EPO and 500 ?mol/L 5-HD,and then deal as asphyxia group.All cells were cultured at 37 ℃ in humidified atmosphere with 50 mL/L CO2 for 24 h.The apoptosis rate of HK-2 cells was detected by flow cytometer.The expressions of Caspase-3 and X-linked inlnibitor of apoptosis protein(XIAP) of HK-2 cells were detected by using immunohistochemical method.Results Compared with control group,after stimulated with postasphyxial-serum,the apoptosis rate and the expression of Caspase-3 of HK-2 cells were significantly increased(Pa
