Effect of Glutamine on Expressions of Nuclear Factor-?B and Heat Shock Protein 70 in Brain of Young Rats Induced by Endotoxin
- VernacularTitle:谷氨酰胺对内毒素诱导幼鼠脑组织核因子-?B和热休克蛋白70表达的影响
- Author:
ya-juan, ZHAO
;
jun, LI
;
mei, SUN
- Publication Type:Journal Article
- Keywords:
glutamine;
endotoxemia;
lipopolysaccharide;
nuclear factor-?B;
heat shock protein 70
- From:Journal of Applied Clinical Pediatrics
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of glutamine(Gln) on expression of nuclear factor kappa B(NF-?B) and heat shock protein 70(HSP70) in brain of young rats induced by lipopolysaccharide(LPS).Methods Ten days old Wistar rats were randomly divided into 3 groups by injection intraperitoneally different agonts,LPS group,normal saline control group(NS group) and Gln group(Gln 1.346 g/kg,1 hour before LPS).NF-?B and HSP70 distribution and expression in brain were deteted by immunohistochemistry.The levels of HSP70 in rats brain induced by LPS were detected by Western blot.SPSS 12.0 software was used.Results The nuclei of neuron in cerebral cortex in LPS group obviously cleared at 6 hours.The positive stain of nuclei in Gln group at 2 hours could not be seen.The stain of nuclei in cerebral cortex was weakened in LPS group at 6 hours by immunohistochemistry.HSP70 protein expression decreased with the measurement of Western blot,especially at 24 hours.HSP70 expression in LPS group was similar as that in NS group.The stain of nuclei in neuron in Gln group at 2 hours increased.It also showed the amount of protein expression increased in Western blot in group Gln at 2,6,12,24 hours(Pa
