Expressions of Chemokine Receptor CXCR3 and Its Ligand Interferon-?-Inducible Protein-10 in Lung Tissue of Asthmatic Model Mice and Their Significances
- VernacularTitle:哮喘小鼠肺组织中CXC趋化因子受体3和其配体干扰素-?诱导蛋白-10的表达及意义
- Author:
liang-xia, WU
;
min, WU
;
xiao-liang, LIN
;
jian-hua, ZHANG
- Publication Type:Journal Article
- Keywords:
asthma;
CXCR3;
interferon-?-inducible protein-10;
dexamethasone;
bacillus Calmette-Guerin vaccine;
mice
- From:Journal of Applied Clinical Pediatrics
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expressions of the chemokine receptor CXCR3 and its ligand IFN-?-inducible protein-10(IP-10) in lung tissue of asthmatic model mice and to explore further the effect of dexamethasone(DEX)and bacillus Calmette-Guerin vaccine(BCG)on the expressions of CXCR3 and IP-10.Methods Forty Kunming mice were randomly divided into 4 groups:asthmatic group,DEX group,BCG group and control group,10 in each group.Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic models.Ten mice in DEX group were administrated DEX (2 mg/kg) by abdomen injection 30 min before challenge (DEX group).Ten mice in BCG group were injected BCG(0.025 mg) intradermally 7,3,and 1 days before sensitization.The mice in control group mice were treated with 9 g/L saline instead of OVA.Mice of each group were executed 24 hours after the final challenge.Their lung tissue were paraffin embedded,sliced and stained by HE.The expressions of CXCR3 and IP-10 protein in lung tissue of mice were detected by immunohistochemistry.Results Distinct differences were discovered in the expressions of CXCR3(F=4.602 P=0.008) and IP-10(F=4.207 P=0.012) among the 4 groups.The expressions of CXCR3 and IP-10 in lung tissue of mice in asthmatic group were significantly higher than those in control group (Pa=0.002),while that in DEX group were significantly lower than that in asthmatic group (P=0.029,0.019).There were no significant difference between the BCG group and asthmatic group.Conclusions The chemokine receptor CXCR3 and its ligand IP-10 play an important roles in mechanisms in the pathogenesis of asthma.DEX and BCG can interfere the expressions of CXCR3 and IP-10 in varying degress.
