Influence of Nano-?-Linolenic Acid on Expression of Macrophage Migration Inhibitory Factor in Viral Myocarditic Mice
- VernacularTitle:纳米?-亚麻酸对病毒性心肌炎小鼠巨噬细胞移动抑制因子表达的影响
- Author:
xiao-hua, YU
;
shuang-jie, LI
;
ping, ZHANG
- Publication Type:Journal Article
- Keywords:
myocarditis,viral;
macrophage migration inhibitory factor;
nano-?-linolenic acid;
mice
- From:Journal of Applied Clinical Pediatrics
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate influence of nano-?-linolenic acid on expression of macrophage migration inhibitory factor (MIF) in murine model with viral myocarditis (VM).Methods Eighty male Balb/c mice were randomly divided into 4 groups equally:control group,model group,low and high dose intervention group.Mice in control group were inoculated introperitoneally with Eagle's solution.Every mouse in other groups was treated with 0.1 mL Coxsackie B3 virus(CVB3) intraperitoneally.Then,mice in low and high dose intervention group were treated with 60 and 180 mg/kg nano-?-linolenic acid solution for 1 week,respectively.Mice in control group and model group were treated with 9 g/L saline for 1 week.All mice were killed on day 15.The expression levels of myocardial MIF mRNA and protein were detected by reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry.Serum MIF concentration were exa-mined by enzymelinked immunosorbent assay(ELISA).Myocardial histopathology was determined with hematoxylin and eosin stain.Results The mortality rate was 0,45%,30% and 20% in control group,model group,low and high dose intervention group,respectively.The mortality rate was significantly lower in high dose intervention group compared with model group (P
