Effect of Erythropoietin on Neuronal Apoptosis and Glycogen Synthase Kinase-3? Expression in Newborn Rats with Hypoxic-Ischemic Brain Damage
- VernacularTitle:促红细胞生成素对缺氧缺血性脑损伤新生大鼠细胞凋亡和糖原合成酶激酶-3?的影响
- Author:
yun-qi, GUO
;
yun-lan, GUO
;
xue-peng, GUO
- Publication Type:Journal Article
- Keywords:
erythropoietin;
hypoxic-ischemic brain damage;
apoptosis;
glycogen synthase kinase-3?;
neonatal rats
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of erythropoietin(EPO)on apoptosis and glycogen synthase kinase-3?(GSK-3?)level in newborn rats with hypoxic-ischemic brain damage(HIBD).Methods Thirty 7-day-old neonatal rats were randomly divided into 3 groups:sham-operated group,hypoxic-ischemic(HI)group and EPO group.Rats in HI group and EPO group were subjected to left common carotid artery ligation,and then they were exposed to 80 mL/L oxygen and 920 mL/L nitrogen gas in the sealed containers of 37 ℃ for up to 2.5 hours to establish HIBD models.EPO was injected intraperitoneally into the EPO group rats after operation,Solution was injected into the first 2 groups.All rats were killed at the 24 hours after operations,The apoptosis was identified and analyzed by flow cytometry.The level of GSK-3? was detected by enzyme-linked immumosorbent assay.Results The neuronal apoptosis and GSK-3? content in the cortex and hippocampus tissue in HI group were significantly higher than those in the sham-operated group(Pa
