Expression of Leukocyte-Function-Associated Antigen-1 on Peripherial Blood Mononuclear Cells in Children with Febrile Seizures
- VernacularTitle:热性惊厥患儿外周血单个核细胞表面淋巴细胞功能相关抗原-1的表达
- Author:
bing, MAO
;
zhi-sheng, LIU
;
hui, YAO
;
dan, SUN
- Publication Type:Journal Article
- Keywords:
febrile seizures;
adhesive molecules;
lymphocyte-function-associated antigen-1
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of leukocyte-function-associated antigen-1(LFA-1)immunoreaction in children with febrile seizures(FS),and explore the neuroimmunomodulation mechanisms in the pathogenesis of FS.Methods Adopting flow cytometry(FCM),the levels of LFA-1 contained in blood serum and peripheral blood mononuclear cells(PBMC)of 60 cases [simple FS(SFS)30 cases;complex FS(CFS)30 cases] with febrile convulsion were analyzed,and compared with those in a normal group(30 cases).Out of 60 children with FS group,the LFA-1 mRNA in 20 cases with SFS and 20 cases with CFS was analyzed,and LFA-1 mRNA in19 health children taken out from control group(30 cases)was analyzed.The real-time PCR was used to detect the expression of PBMC LFA-1 mRNA.Results The expression of LFA-1 in the surface of PBMC of the 3 groups,the highest LFA-1 level was in the SFS group(50.89?21.36),the lowest LFA-1 level was in the CFS group(34.35?11.45),and control group was(41.39?16.30).Significant differences were found in 3 groups(Pa
