Effects of Glycogen Synthase Kinase-3? and Free Radical on Neuron Apoptosis of Newborn Rats with Hypoxic-Ischemic Brain Damage
- VernacularTitle:糖原合成酶激酶-3?和自由基在缺氧缺血性脑损伤新生大鼠细胞凋亡中的作用
- Author:
wei-hua, DONG
;
li-na, TAN
;
xue-peng, GUO
- Publication Type:Journal Article
- Keywords:
hypoxic-ischemic brain damage;
synthasekinase-3?;
free radical;
apoptosis;
neonatal rats
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of glycogen synthase kinase-3?(GSK-3?)and free radical on neuron apoptosis of hypoxic-ischemic brain damage(HIBD)in newborn rats.Methods Eighty 7-day-old neonatal rats were randomly divided into 2 groups:normal group and hypoxic-ischemic(HI)group.Rats in HI group were subjected to left common carotid artery ligation,exposed to 80 mL/L oxygen and 920 mL/L nitrogen gas in 37 ℃ closed container for 2.5 h.Rats in 2 groups were killed at 6 hours,24 hours,48 hours,72 hours,5 days after hypoxia respectively.The neuron apoptosis was detected by flow cytometry.The activity of GOD-PX and the contents of SOD and MDA were detected by spectrophotometry,the level of GSK-3? was mensurated by Enzyme-linked immumosorbent assay(ELISA).Results The rates of neuronal apoptosis in HI group were significantly higher than those in normal group at 6,24,48 and 72 hours after hypoxia,respectively(Pa
