Application of Serum Protein Markers in Hirschsprung′s Disease
- VernacularTitle:血清蛋白质标记物在先天性巨结肠患儿中的应用
- Author:
xin-rang, CHEN
;
jia-xiang, WANG
- Publication Type:Journal Article
- Keywords:
Hirschsprung′s disease;
diagnosis;
surface enhanced laser desorption/ionization time of flight mass spectrometry;
support vector machine;
protein fingerprint
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To check serum protein of children′s Hirschsprung′s disease(HD) and sift specific protein marker which was used in constructing of HD screening and early diagnosis of serum protein fingerprint model.Methods Surface-enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS) was applied to detect protein mass spectrometry of serum specimens in 82 cases(HD group 42 cases,20 cases of other types of obstruction,healthy control group 20 cases) and data were analyzed by bioinformatics methods(support vector machine).Results 1.For HD group and healthy control group:selected 3 M/Z in 3 221.7,5 639.2,6 884.2 protein markers were selected,HD early screening and diagnostic model was established,3 markers in HD low expression,the expressions of them in HD group and healthy control group were 378.29?273.34,295.65?159.38,444.13?254.06 and 1 428.18?1 192.61,1 039.60?785.64,1 115.72?680.48,respectively.There were significant differences in two groups(Pa0.05).Conclusions The establishment of serum protein fingerprint model of HD by SELDI-TOF-MS support vector machine could screen and diagnose HD early,which is a new method of better specificity,high sensitivity and is worthy of further research and application.
