Influence of Inhaled Glucocorticosteroid on ?-Glutamylcysteine Synthetase in Inflammatory Cell of Sputum in Children with Asthma
- VernacularTitle:吸入糖皮质激素对哮喘患儿痰炎性细胞?-谷氨酰半胱氨酸合成酶的影响
- Author:
cong-yu, DAI
;
yun-fu, ZHU
;
yong-liang, JIANG
;
chen-tao, LIU
- Publication Type:Journal Article
- Keywords:
bronchial asthma;
?-glutamylcysteine synthetase;
child
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of inhaled glucocorticosteroid on ?-glutamylcysteine synthetase(?-GCS) in inflammatory cell of sputum in children with asthma.Methods Twenty-two asthmatic children were divided into 2 groups according to treatment.The children who were treated by inhaled budesonide combined with salbutamol were due to group A and the others inhaling salbutamol only were due to group B,the healthy children were acted as healthy control group(group C).The glutation(GSH),total GSH and the activity of ?-GCS in sputum were measured respectively;Expression of ?-GCS in inflammatory cell of sputum were detected by immunohistochemistry;the expression of ?-GCS heavy chain(?-GCS-h) mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results 1.The total GSH[(1.08?0.14) ?mol/L] and oxidized glutathione(GSSG)[(0.37?0.09) ?mol/L] were decreased in sputum of group A of post-treatment compared with pre-treatment(Pa
