Changes of Expression and Activity of Heme Oxygenase-1 in Lung Tissue of Hyperoxia-Induced Preterm Rats
- VernacularTitle:高体积分数氧暴露早产大鼠肺组织血红素加氧酶-1的表达及活性变化
- Author:
yu-feng, QUAN
;
ming-ci, ZHENG
;
hua, ZHANG
;
yi, DENG
;
hong, ZHANG
- Publication Type:Journal Article
- Keywords:
hyperoxia;
lung;
heme oxygenas;
preterm rat
- From:Journal of Applied Clinical Pediatrics
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of expression and activity of heme oxygenase-1(HO-1) in lung tissue of preterm rats exposed to hyperoxia,and explore the role of HO-1 in hyperexia-induced lung injury of preterm rats.Methods Three-day-old preterm rats of standard SD were randomly assigned to hyperoxia group and air group.At the third and 7th day of exposure,the expressions of HO-1 mRNA were detected by means of reverse transcription polymerase chain reaction and the cellular distribution and expressions of HO-1 protein in the lung were measured by immunohistochemical techniques,respectivesly,and the active of HO-1 was determined also.Results On the third day,in air group,the expressions of HO-1 mRNA(0.17 ?0.08),HO-1(7.23?4.63)were mildly expressed and the activity of HO-1 was(4.32?1.57) nmol/(mg?h);compared with those of air group,the expression of HO-1 mRNA in hyperoxia group(0.72?0.33) was significantly increased(Pa
