Relationship between ?-Actinin-4 and Oxidative Stress in Rats with Doxorubicin Nephropathy
- VernacularTitle:?-actinin-4与多柔比星肾病大鼠氧化应激状态的关系
- Author:
min, ZHANG
;
ying, ZHU
;
bo, HU
;
ling, LU
- Publication Type:Journal Article
- Keywords:
?-actinin-4;
urine protein;
oxidative stress;
Benazepril;
rat
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the relationship between ?-actinin-4 and oxidative stress in rats with Doxorubicin nephropathy,and to conjecture the mechanism of Benazepril reducing urine protein.MethodsThe model was established by a single intravenous injection of Doxorubicin(5 mg/kg).Benazepril[6 mg/(kg?d)] was orally administered to medicine-treated group.Normal control group received the same volume of 9 g/L sodium chloride solution.Maleic dialdehyde(MDA),superoxide dismutase(SOD),total antioxygen capability(T-AOC) were detected by kit.Sterptavidin biotin peroxidase complex(SABC) immunohistochemistry were used to observe the expression and distribution of ?-actinin-4 in rat glomeruli.ResultsSeven days after injection of Doxorubicin,the decrement of the activity of T-AOC,the progressive increase of expression of ?-actinin-4 whose distribution was in derangement in experimental group.During 14 to 28 days,compared with normal control group,the urine protein,MDA increased significantly,while SOD decreased in experimental group(Pa
