Expressions of Hepatocyte Growth Factor and Its Receptor in Children with Primary Nephrotic Syndrome
- VernacularTitle:原发性肾病综合征患儿肝细胞生长因子及其受体的表达特征
- Author:
hong, LIU
;
bao-jin, HU
;
rui, FU
;
yin, ZOU
;
jiang-wei, KE
- Publication Type:Journal Article
- Keywords:
hepatocyte growth factor;
c-Met;
nephrotic syndrome;
tubulerinterstitial lesions;
child
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the expressions of hepatocyte growth factor(HGF) and its receptor c-Met in renal tissues of children with primary nephrotic syndrome(PNS) and the changes of serum HGF,and to explore its role in PNS chronic progress.MethodsForty-five children with PNS in active stage were studied.Among them,5 cases had severe tubulointerstitial lesions,12 cases had moderately tubulointerstitial lesions,21 cases were mild,7 cases without lesions.Serum from 20 normal cases and 10 normal renal tissues were evaluated as well.Inter-group comparison using One-Way ANOVA.Enzyme linked immunosorbent assay(ELISA) was used to examine the serum HGF,and immunohistochemistry staining and image analysis methods were used to study the expressions of HGF,c-Met,transforming growth factor-?1(TGF-?1) and ?-smooth muscle actin(?-SMA) in renal tissues.ResultsThe levels of HGF and c-Met protein expressions in renal tissues of children with severe tubulointerstitial lesions were significantly lower than those in the mild group and moderate group(Pa0.05).The level of HGF expression had positive correlation with the levels of TGF-?1,?-SMA among children with mild and moderately renal tubulointerstitial lesions(r=0.521,0.603Pa
