Relationships of Vascular Endothelial Growth Factor and Interferon-?,Interleukin-4 in Asthmatic Rats and Effect of Budesonide on Their Expression
- VernacularTitle:支气管哮喘大鼠血管内皮生长因子与?-干扰素、白细胞介素-4变化的相关性及布地奈德干预对其的影响
- Author:
xiao-hong, JIN
;
chang-chong, LI
;
cun-guo, CHEN
;
shao-bo, LI
;
feng-xian, LI
;
ling-hong, GAN
- Publication Type:Journal Article
- Keywords:
asthma;
vascular endothelial growth factor;
interferon-?;
interleukin-4;
Budesonide;
rat
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of vascular endothelial growth factor(VEGF),IFN-? and IL-4 and effect of Bude-sonide on their in asthmatic rats.Methods Thirty-six male SD rats were randomly divided into 3 groups:asthma group,Budesonide-treatment group and control group.On the first day of the experiment and the 8th day,the rat models of the asthma group and Budesonid treatment group were allergized by the OVA/Al(OH)3 through intraperitoneal injection,respectively.And starting from the 15th day,they were challenged by the OVA through atomization for 2 weeks.Control group was allergized and challenged by NS atomization.Budesonid treatment group was interfered in Budesonide inhalation before suscitation in 0.5 h.After 12 h the same inhal done was again in Budesonide group.Twenty-four hours after the last challenge,the rats in 3 groups were sacrificed,and blood and bronchoalveolar lavage fluid(BALF) were collected.The concentrations of IL-4,IFN-? and VEGF in serum and BALF were measured by enzyme-linked immunosorbent assay.Results The concentrations of IL-4 in serum and BALF in asthma group and Budesonide treatment group were significantly increased than those in control group(P
