Observation of Testis Tissue in Experimental Cryptorchidism after Orchiopexy in Various-Day-Rats
- VernacularTitle:不同日龄隐睾复位大鼠睾丸组织结构观察
- Author:
cui-ping, SONG
;
hai-lei, WANG
;
guo-lin, ZHANG
;
fang, AI
;
jin-lan, CHEN
- Publication Type:Journal Article
- Keywords:
cryptorchidism;
germ cell;
cell apoptosis;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe testis tissue changes of experimental cryptorchidism after orchiopexy in various-day-rats.MethodsSeventy-two Sprague-Dawley(SD) male rats were divided into 3 groups randomly and made artificial cryptorchidism:unilateral cryptorchidism group(n=24),bilateral cryptorchidism group(n=24)and sham operation group(n=24)at age 21-day-old.Intra-abdominal testicle was resetting in 2 weeks,at age 40 days and 60 days,the rats were sacrificed for detection tubular fertility index(TFI) and mean tubular diametar(MTD) with hematoxylin-eosine staining and germ cell apoptosis by terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling(TUNEL) assay.ResultsThere were significant differences of MTD,TFI and apoptosis index(AI) between cryptorchidism and scrotal testes(P0.05).The AI of cryptorchidism testes of unilateral cryptorchidism group were significantly lower than that of bilateral cryptorchidism group in 40 days(P0.05).ConclusionsAI of artificial resetting testis is increased and contralateral descended testes in unila-teral cryptorchidism have various damage.It is to lighten that pathological damage of testes of cryptorchidism with prolongation of reset time.
