Inhibitory Effect of Silybin-Phosphatidylcholine Compound on Production of Nitric Oxide and Reactive Oxygen Species in Mouse Macrophage
- VernacularTitle:水飞蓟宾-磷脂酰胆碱复合物对小鼠巨噬细胞一氧化氮及活性氧生成的抑制作用
- Author:
yun-qi, GUO
;
yun-lan, GUO
;
xin-hui, LIU
;
li-hong, PEI
- Publication Type:Journal Article
- Keywords:
silybinin;
silybin-phosphatidycholine compound;
inducible nitric-oxide synthase;
nitric oxide;
reactive oxygen species
- From:Journal of Applied Clinical Pediatrics
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of silybin-phosphatidylcholine compound (SPC) on H2O2-induced the production of nitric oxide (NO) and reactive oxygen species (ROS) in mouse macrophage.Methods Macrophage were collected in abdominal cavity of 6-8 week Kunming mouse,and cultured macrophage(2?108 L-1) were divided into control and SPC group randomly.Macrophage in control group were added into the same volume(0.1 mL) of 9 g/L sodium chloride.Macrophage in H2O2 group were added into a single bolus of H2O2 (1 mmol/L H2O2) for 30 min,while macrophage in SPC group were preincubated with different concentration of SPC for 2 h followed by a 30 min incubation with 1 mmol/L H2O2.Immunocytochemistry were used to measure the contents of inducible nitric-oxide synthase(iNOS),NO production was determined by Griess reactive, ROS was determined by DCFH-DA Fluorescence probe.Results The production of NO in H2O2 group markedly higher than that in control group(P
