Influence of Nuclear Factor-?B on Cardiac Myocyte Apoptosis in Septic Shock Mouse
- VernacularTitle:核因子-?B对感染性休克小鼠心肌细胞凋亡的影响
- Author:
hui-fang, HOU
;
jian-zhi, GAO
;
lin-yu, WEI
;
zheng-yue, CHEN
;
yong-ling, WANG
- Publication Type:Journal Article
- Keywords:
septic shock;
nuclear factor-?B;
myocyte apoptosis;
mice
- From:Journal of Applied Clinical Pediatrics
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of nuclear factor-kappaB(NF-?B) on cardiac myocyte apoptosis and understand the molecular mechanism of decrease of heart function in septic shock rats.Methods Twenty Wistar rats were randomly divided into the septic shock group and the normal control group.The septic shock group(n=10) were fixed with anaesthesia and made into 1.5 cm slices just along the abdomen midline.Then the roots of appendices were returned to the belly cavity after being ligated and punctured 4 times with the No.14 pinhead.After that,the slices were sewn up layer by layer.After 12 hours,the rats were all sacrificed,the blood taken and the serum separated.In the end,the heart specimens of the septic shock group were set aside.Meanwhile,the normal control group were dealt with in the same way except that they were not subjected to cecal ligation and puncture.Then NF-?B protein levels in cardiac tissue and the index of cardiac myocyte apoptosis were measured.Results NF-?B protein levels in the septic shock group(9 cases were strong masculine gender,1 case was middle masculine gender)elevated significantly compared with the normal control group(8 cases were negative gender,2 cases were weak masculine gender) in cardiac tissue(P
