Intervention Effect of Silymarin Attenuate Tubulointerstitial Fibrosis in Rats with Unilateral Ureteral Obstruction
- VernacularTitle:水飞蓟素对单侧输尿管梗阻大鼠肾小管间质纤维化的干预作用
- Author:
zi-ming, HAN
;
de-an, ZHAO
;
hai-xia, ZHAO
- Publication Type:Journal Article
- Keywords:
silymarin;
unilateral ureteral obstruction;
tubulointerstitial fibrosis;
transforming growth factor-?1;
collagen type Ⅲ;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of silymarin on expressions of transforming growth factor-?1(TGF-?1) and collagen type Ⅲ(Col Ⅲ) in rats with experimental tubulointerstitial fibrosis(TIF) induced by unilateral ureteral obstruction(UUO).Methods Se-venty-two Sprague-Dawley rats were randomly divided into 3 groups:sham operation group(n=24),model group(n=24) and silymarin treatment group(n=24).The rats in model group and treatment group were operated by ligation of left-ureter.The rats in sham opera-tion group operated by dissociating left-ureter were taken as controls.Rats in treatment group were fed with silymarin [30 mg/(kg?d)]24 h before operation,and rats in model group and sham operation group were treated by intragastric administration with the same volume nomal saline at the same time.Eight rats were killed at day 7,14,and 21,respectively.The score of TIF changes,histologically,was evaluated under light microscope.Expressions of TGF-?1 and Col Ⅲ in tubulointerstitial tissue in 3 groups were investigated by immunohistochemistry.Results 1.The TIF pathological index in treatment group was less than that in model group,but more than sham operation group at day 14 and 21(Pa
