Effect of Momordicin on Expression of Tumor Necrosis Factor-? in Balb/c Mice with Viral Myocarditis Caused by Coxsackievirus B_3
- VernacularTitle:苦瓜素对柯萨奇B_3病毒所致病毒性心肌炎小鼠肿瘤坏死因子-?表达的影响
- Author:
hong, TIAN
;
xiao-ming, LI
;
shuang-jie, LI
- Publication Type:Journal Article
- Keywords:
momordicin;
myocarditis;
virus;
tumor necrosis factor-?;
mice
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of momordicin on tumor necrosis factor-?(TNF-?) level,mRNA transcription,and protein expression in myocardium of viral myocarditis caused by coxsackievirus B3(CVB3),and explore its therapeutic mechanism on viral myocarditis in Balb/c mice.Methods Fifty Balb/c mice were randomly divided into 3 groups as follows:momordicin treatment group(20 cases),vehicle control group(20 cases) and normal control group(n=10).Mice in the vehicle control group and the momordicin treatment group were intraperitoneally inoculated with CVB3,as for the nomal control group,equal amount of culture fluid was given instead.Momordicin[25 mg/(kg?d)] was administered intraperitoneally daily from day 0 to 6.Myocardial histopathology,cardiac TNF-? antigen,protein and mRNA expression were detected on day 15 after CVB3 inoculation,respectively.Results As compared with model group,in mice treated with momordicin,the histological myocardial lesion was significantly reduced [(3.26 ?0.84) vs(1.56?0.48),t=3.90 P
