Expression of Hepatocyte Growth Factor and Transforming Growth Factor-? of Renal Tissues in Children with Nephropathy
- VernacularTitle:肝细胞生长因子与转化生长因子-?在肾脏病患儿肾组织中的表达
- Author:
ji-yang, YU
;
guang-hua, ZHU
;
qing, SHI
;
wei-xun, HE
- Publication Type:Journal Article
- Keywords:
hepatocyte growth factor;
transforming growth factor-beta;
glomerular sclerosis;
focal segmental glomerulosclerosi;
child
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and mutual relationship between transforming growth factor-?(TGF-?) and hepatocyte growth factor(HGF) in children's renal tissues,as well as the effect of the 2 indexes on the renal pathological change.Methods According to the severity of renal pathological change under light microscope,61 cases were divided into 3 groups:named control group(26 cases,clinically diagnosed as thin basement membrane nephropathy),test group Ⅰ [22 cases,clinically diagnosed as less evident focal segmental glomerulosclerosi(FSGS) nephropathy] and test group Ⅱ(13 cases,clinically diagnosed as evident FSGS nephropathy).Immunity class test(SP method:streptavidinbiotin peroxidase method) was used to detect the representation of HGF and TGF-?.Semi-quantitative analysis had been carried out in all cases.Film reading of cell was viewed by Olympus microscope,brown yellow from cytoplase as the positive signal,10 high power microscope visions were randomly selected from renal glomerali area and 10 from renal interstitium area.Medical image analysis software was used to determine the masculine area of HGF or TGF-? and image intensity;then the immunity class index was defined as masculine area ? image intensity.Results 1.HGF and TGF-? existed in all renal tissues;2.Expressions of HGF and TGF-? increased obviously along with FSGS pathology alteration(Pa