Temporal Expressions and Significances of Matrix Metalloproteinases-13 and Tissue Inhabitor of Metalloproteinases-1 in Lung of Newborn Rats with Hyperoxia Induced Chronic Lung Disease
- VernacularTitle:高体积分数氧致慢性肺疾病新生大鼠肺组织基质金属蛋白酶-13和基质金属蛋白酶组织抑制剂-1表达的意义
- Author:
ning, CHEN
;
xue-yan, LIU
;
lei, NA
;
xin-dong, XUE
- Publication Type:Journal Article
- Keywords:
chronic lung disease;
hyperoxia;
collagen Ⅰ;
matrix metalloproteinases-13;
tissue inhibitor of metalloproteinases-1
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe temporal expression of matrix metalloproteinases(MMP-13) and tissue inhibitor of metalloproteina-ses-1 (TIMP-1) in lung of newborn rats with hyperoxia induced chronic lung disease (CLD),and to explore the relationship of CLD with MMPs.Methods The neonatal rats within 24 hours after birth were randomly divided into hyperoxia-exposed group(n=40) and control group(n=40).On postnatal 1,3,7,14 and 21 days,lung tissue of rats in 2 groups were collected.Lung histological changes were evaluated by hematoxylin and eosin and Masson stain;Collagen Ⅰ was detected by enzyme linked immunoadsorbent assay;MMP-13 and TIMP-1 were identifide by immunohistochemistry.Results Exposured to hyperoxia enviroment for 21 days,the number of alveolar decreased,terminal air space enlarged,inter-alveolar septa thickened,and deposition of interstitial collagen fibers.On 14 and 21 days,collagen Ⅰ in the lung of hyperoxia-exposed group increased significantly compared with that of control group(P0.05),obviously decreased on 21 day(P
