Effect of Bromocriptine on the Activation of T Lymphocyte Stimulated by Phytohemagglutinin
- VernacularTitle:溴隐亭对植物血凝素诱导活化T淋巴细胞的影响
- Author:
ying, SHI
;
lan-zhi, MAO
;
bao-rui, YU
;
zhi-jun, HU
;
jing-jing, ZHANG
;
zhi-tao, GAO
;
hui, WANG
- Publication Type:Journal Article
- Keywords:
bromocriptine;
ZAP-70;
linker for activation of T cell;
prolactin;
nuclear factor-?B
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of bromocriptine(BRC) on the activation of T lymphocyte stimulated by phytohemagglutinin(PHA).Methods After CD4+ T cell line Jurkat E6-1 cells were stimulated by PHA,prolactin(PRL) and BRC,respectively,the expression of linker for activation of T cells(LAT) and zeta-chain T cell receptor associated protein kinase 70 000(ZAP-70) mRNA of T lymphocytes were checked by RT-PCR.The expression of PRL mRNA of T lymphocytes was detected by Real time PCR.The expression of CD25(cluster of differentiation) as a marker of early activation on the surface of T lymphocytes was detected by flow cytometry,and the activation of nuclear factor-?B(NF-?B) was detected by luciferase reporter system.Results 1.BRC inhibited the expression of ZAP-70 as the common signal molecules both in the T lymphocyte activation pathway and PRL-prolactin-prolactin receptor(PRLR) signal transduction pathway,and decreased the expression of PRL mRNA produced by activation T lymphocytes.2.BRC enhanced the expression of LAT mRNA as another important signal molecular on the T lymphocytes and CD25 on the surface of the T lymphocytes.3.The activation of NF-?B of T lymphocytes was decreased.Conclusions BRC might inhibit the activation of T lymphocytes by inhibiting the expression of ZAP-70,the common signal molecules between T lymphocytes activation and PRL-PRL pathway,and PRL mRNA,the like-T lymphocyte growth factor.
