Expressions of Synaptophysin in Temporal Lobe Cortex and Hippocampus of Rat with Epilepsy Induced by Pentylenetetruzole and Interventive Effect of Gastrodin
- VernacularTitle:戊四氮致癎幼鼠颞叶和海马区突触体素表达及天麻素对其的干预作用
- Author:
ya-qin, CAO
;
yi-fan, SU
;
hong, CHEN
;
jiao, DONG
;
jian-ping, WANG
- Publication Type:Journal Article
- Keywords:
epilepsy;
pentylenetetrazole;
synaptophysin;
synaptic reconstruction;
gastrodin
- From:Journal of Applied Clinical Pediatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the behavior changes and synaptic reconstruction of brain and the impact of gastrodin in rats with epilepysy induced by pentylenetetrazole.Methods Fifty Wistar rats in growth(50-70 g) were randomly divided into 5 groups:control group,Pentylenetetrazole (PTZ) group,gastrodin high dose group,gastrodin low dose group and sodium valproate group,each group had 10 rats.According to Racine classification,the behavior changes of rats and the frequency were recorded.Conventional method was adopt to perfuse cordis, fix and extract the brain,and the immunohistochemistry was used to detect synaptophysin(P38) expression in the temporal lobe and hippocampus of the brain after 4 weeks.Results 1.Four weeks after pentylenetetrazole ignited the growth period rats,the attack-level of each experimental group through comparison with each other,there was statistically significant difference(?2=35.83 P0.05).Conclusions There are mossy fiber sprouting and the formation of synaptic reconstruction in temporal lobe and hippocampus of the growth period rats repea-tedly ignited by pentylenetetrazole.Gastrodin may play a role in the formation of antiepileptic obviously,and through decreasing the expression of P38,which can inhibit the formation of synaptic reconstruction,as control seizures indirectly.
