Effect of Postasphyxial-Serum in Neonate on Expression of Omi/HtrA2 in Renal Tubular Cells
- VernacularTitle:窒息新生儿血清对肾小管上皮细胞内Omi/HtrA2表达的影响
- Author:
yong, ZHANG
;
wen-bin, DONG
;
cun-liang, DENG
;
ming-yong, WANG
;
feng, CHEN
;
kai-gui, XU
- Publication Type:Journal Article
- Keywords:
infant,newborn;
asphyxia;
serum;
apoptosis;
Omi/HtrA2
- From:Journal of Applied Clinical Pediatrics
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of postasphyxial-serum in neonate on expression of serine protease Omi/HtrA2 in renal tubular cells(HK-2).Methods Human renal proximal tubular cell line HK-2 cell was used as target cell.The cultural cells in orifice were divided into control group and asphyxia-serum attacking group.Blood was cowected from asphyxia newborns by means of femoral venous puncture,then the serum was garthered,anticoagulated by liquemie,3 000 r/min centrifuged 20 min,abstracted serum,thermostatic waterbathed the serum at 56 ℃,so that to inactivate addiment,filtered germ by micropore filte,the attacking concentrtion of serum was 200 mL/L,the cells of the asphyxia-serum attacking group were attacked by asphyxia-serum,and the cells of control group were cultivated with normal nutritive medium when the cells was needed.After 24 hours,the cells were tixed,then the expression of Omi/HtrA2 in cytoplast was detected by the use of immunohistochemical method.Results Omi/HtrA2 was inaurate or yellow brown and localized to the cytoplast.The rate of the cell expressed Omi/HtrA2 was(9.0?2.5)% in control group,after stimulated with postasphyxial-serum,in asphyxia group the rate of the cell expressed Omi/HtrA2 was(25.15?3.5)%,there was significant difference between 2 groups(t=-15.322 P
