Expression of Basic Fibroblast Growth Factor in Rats Model of Doxorubicin Induced Nephropathy
- VernacularTitle:碱性成纤维细胞生长因子在多柔比星肾病大鼠模型中的表达
- Author:
li-min, WANG
;
xue, YANG
;
lei, NIE
;
ying, LI
- Publication Type:Journal Article
- Keywords:
doxorubicin;
basic fibroblast growth factor;
proteinuria;
immunohistochemistry;
rat
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and importance of basic fibroblast growth factor(bFGF)in renal tissue of doxorubicin-induced nephropathy rats,and discuss its possible role in minimal change nephrotic syndrome(MCNS).Methods Fifty male Wistar rats were enrolled in this study.They were randomly divided into normal group(n=10)and nephropathic group(n=40).The nephropathic group was established by a single intraperitoneal injection of doxorubicin 5 mg/kg.The rats of nephropathic group were killed on the 3rd,7th,14th and 28th day and 24 h urinary protein of all rats was measured.Normal group rats were killed on the 28th day.bFGF level in renal tissue was determined with immunohistochemistry assays,and was quantitatively analyzed with color image analysis system.The SPSS 10.0 software was used to obtain t-test of various groups and correlation analysis of 2 variables.Results 1.The 24 h urinary protein of nephropathy rats increased gradually on the 7th,14th and 28th day,and it was greatly higher than that in control group(Pa
