Expression of Tenascin during Heart Development in Rats
- VernacularTitle:韧黏素在大鼠胚胎心脏发育过程中的表达
- Author:
xue-ying, CHENG
;
jun, LI
;
da-wei, WANG
- Publication Type:Journal Article
- Keywords:
tenascin;
heart;
embryonic development;
rat
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of tenascin-C(TN-C)and tenascin-X(TN-X)in the embryonic heart of rats,investigate their role during the heart development.Methods Sixty-four Spraque-Dawley female rats and 20 male rats were put into the same cage,when vaginal suppository was found,the femate was pregnant 0.5 day(ED 0.5),then executed the pregnant rats on ED 12.5,ED 15.5 and ED 19.5,got out the hearts.Semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detect the TN-C and TN-X mRNA expression in the rat hearts aged embryonic day 12.5(ED 12.5),ED 15.5 and ED 19.5.The protein expression of TN-C and TN-X in the embryonic hearts was detected by immunohistochemistry.Results TN-C mRNA was highly expressed in ED 12.5 embryonic hearts(0.683 7?0.043 1),the expression of TN-C mRNA of ED 15.5(0.454 3?0.038 8)and ED 19.5(0.250 5?0.038 4)embryonic hearts decreased,they were different from the expression of ED 12.5(Pa
