Angiotensin Ⅱ Stimulate the Expression of Integrin-Linked Kinase in Rat Glomerular Mesangial Cell
- VernacularTitle:血管紧张素Ⅱ上调大鼠肾小球系膜细胞整合素连接激酶的表达
- Author:
zhi-hui, LI
;
tian-hui, WU
;
cui-rong, DUAN
;
jin-hua, HE
;
yan, YIN
- Publication Type:Journal Article
- Keywords:
mesangial cell;
rat;
integrin-linked kinase;
angiotensin Ⅱ
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of angiotensin Ⅱ(Ang Ⅱ)on the expression of integrin-linked kinase(ILK)in rat glomerular mesangial cells.Methods SD rat glomerular mesangial cells were separated and cultured.They were treated with 10-7 mol/L Ang Ⅱ in various time-point(0,12,24,48,72 h),and then cultured rat glomerular mesangial cells were treated 48 h with Ang Ⅱ in various concentration(0,10-11,10-9,10-7,10-5 mol/L).Then total protein expression in rat glomerular mesangial cells stimulated by Ang Ⅱ at every time-point and every concentration-point was extracted.The expression of ILK protein was measured by Western blot.Results 1.Expression of ILK protein in rat glomerular cells stimulated by Ang Ⅱ in various time-point,or various concentration was significantly increased.In group of 10-7 mol/L Ang Ⅱstimulated cells,the protein semiquantity of ILK at 0,12,24,48,72 h respectively was 0.18?0.02,0.37?0.07,0.90?0.10,1.73?0.12,and 1.72?0.13(F=166.78 P
