Expression of ?-Smooth Muscle Actin in Glomerulosclerosis Rats and Its Relationship with Renal Function
- VernacularTitle:肾小球硬化大鼠肾脏组织?-平滑肌肌动蛋白的表达特点及其与肾功能的关系
- Author:
juan, PEI
;
yuan-han, QIN
;
feng-ying, LEI
;
peng, HU
- Publication Type:Journal Article
- Keywords:
glomerulosclerosis;
?-smooth muscle actin;
daunorubicin;
renal function
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of ?-smooth muscle actin(?-SMA)in glomerulosclerosis rats and its relationship with renal function.Methods Forty healthy Wistar rats were equally divided into 2 groups including sham operated group and model control group.Rats in model groups were uninephrectomized and injected with daunorubicin(5 mg/kg)after 1 week through the tail vein.Twenty-four hours of urinary protein excretion,serum creatinine(Scr),blood urea nitrogen(BUN)were measured at the 12th week.Renal pathology was evaluated.Immunohistochemistry(SupervisionTM)was performed on renal glomeruli tissue to detect the expression of ?-SMA.Reverse transcription polymerase chain reaction(RT-PCR)was used to examine the expression levels of ?-SMA mRNA in glomeruli.SPSS 13.0 software was used to analyze the two variables.Results In model control group,the urinary protein,Scr,BUN significantly increased(Pa
