Effect of Bacillus Calmette-Guerin Intervention on Activation and Apoptosis of T Lymphocyte in Asthmatic Mouse
- VernacularTitle:卡介苗干预对哮喘小鼠T淋巴细胞活化/凋亡状态的影响
- Author:
rong-ling, GE
;
jian-hua, ZHANG
;
lun, XU
- Publication Type:Journal Article
- Keywords:
bacillus calmette-guerin;
costimulatory molecule;
activatation;
apoptosis
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of bacillus calmette-guerin (BCG)intervention on expression of costimulatory molecules of B7-CD28/CD152 and T lymphocyte apoptosis in asthmatic mouse.Methods Twenty-seven mice were randomly divided into:asthma model group,BCG treatment group and normal control group.The mice were sensitized by ovalbumin(OVA) and 100 g/L Al(OH)3 with intraperitoneal injection,challenged with atomization inhalation,then reproduced the asthmatic models.The BCG treatment group were treated with BCG(0.025 mg,merely) subcutaneous injection 3 times before sensitization(5,3,1 day).The normal control group were treated with 9 g/L saline water taking place of OVA and Al(OH)3.CD28,CD152,CD80 and CD86 were analyzed by flow cytometry in splenic lymphocyte suspension.T lymphocyte was cultivated from peripheral blood mononuclear cell.The apoptosis ratio of T lymphocyte was counted by Comet Assay.SPSS 10.0 software was applied to analyze data.Results Contrasting asthma model group with normal control group,CD28 was up regulated(t=3.94 P0.05),CD86 was up regulated(t=2.68 P0.05).Contrasting BCG treatment group with asthma model group,T lymphocyte apoptosis rate was higher(t=3.15 P
