Expressions of Silencer of Death Domains and p65 in Children with Acute Lymphoblastic Leukemia and Its Relationship with Chemotherapeutic Drugs
- VernacularTitle:死亡结构域沉默子和p65在儿童急性淋巴细胞白血病中的表达及与化疗的关系
- Author:
hong-fang, TAO
;
qun, HU
;
jian-lin, FANG
;
ai-guo, LIU
;
shuang-you, LIU
;
liu-qing, ZHANG
;
ying, HU
- Publication Type:Journal Article
- Keywords:
leukemia,lymphocytic,acute;
siencer of death domains;
phospho-nuclear factor-?B-p65;
tumour necrosis factor receptor-1
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of silencer of death domains(SODD) and its clinical significance and relationship with phospho-NF-?B-p65 proteins in bone marrow cells of acute lymphoblastic leukemia(ALL)in children,and the expression of SODD and phospho-NF-?B-p65 in Jurkat cells treated with chemotherapeutic drugs in order to find a new chemotherapeutic target.Methods The expressions of SODD and phospho-NF-?B-p65 proteins in bone marrow cells were detected by immunohistochemistry in 25 children with ALL.The apoptosis incidence was measured by Annexin-V-Fluorescence/PI double-labeling flow cytometry and the expression of SODD and phospho-NF-?B-p65 proteins were determined by Western blotting in Jurkat cells.Results It was found that the expression of SODD and active p65 expression in ALL were significantly higher than those in healthy control group.The expression of SODD and phospho-NF-?B-p65 proteins in the high-risk(HR) group was significantly higher than those in standard-risk(SR) group(Pa
