Expression of Thyroid Hormone Receptor Interacting Protein 3 in Children with Acute Lymphocytic Leukemia and Its Significance
- VernacularTitle:甲状腺激素受体结合蛋白3基因在儿童急性淋巴细胞白血病中的表达及意义
- Author:
xiao-hui, LI
;
jian-bai, ZHANG
;
fang, NING
;
jing, LI
- Publication Type:Journal Article
- Keywords:
thyroid hormone receptor interacting protein 3;
leukemia,lymphocyte,acute;
reverse transcription-polymerase chain reaction;
child
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of the remission in children with acute lymphocyte leukemia(ALL),as well as the expression of thyroid hormone receptor interacting protein 3(TRIP3) gene in children with ALL and explore the relationship between TRIP3 and ALL.Methods Fasting venous blood 2-4 mL was collected,anticoagulanted with ethylenediamine tetraacetic acid(EDTA),then perpheral blood mononuclear cells was collected by Ficoll density gradient centrifugation,total RNA was extracted by Trizol one step method.Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect TRIP3 expression in peripheral blood lymphocytes in 73 ALL children of different stages and 20 normal children.The relationship between TRIR3 expression in children and ALL release was analyzed.Results 1.Expression of TRIP3 was significantly lower both after initial treatment and during recrudescence than that in normal children(Pa0.05).3.In children with ALL after initial treatment,the remission rate was significantly higher in TRIP3 positive patients than in TRIP3 negative ones(remission rate discern 25.0% vs 84.2%,P
