Reversing Mechanism of Adriamycin Resisitance of K562/A02 Cell Line by Ciclosporin through H2AX Suppression to Attenuate DNA Damage Repair
- VernacularTitle:环孢素通过抑制H2AX表达减少DNA损伤后修复逆转K562/A02细胞对多柔比星的耐药性
- Author:
fen, ZHOU
;
qiu-ling, WU
;
xian, LU
- Publication Type:Journal Article
- Keywords:
ciclosporin;
deoxyribonucleic acid double strands break;
K562/A02 cell line;
adriamycin;
drug resistance;
leukemia
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the reversing mechanism of multidrug resistance of ciclosporin(CsA) on K562/A02 cell line,attenuating DNA damage repair through H2AX suppression.Methods K563 and K562/A02 respectively co-cultured with adriamycin(ADM) of different concentrations and CsA.MTT assay was employed to determine the inhibitory concentration of 50 percent(IC50),the resistance times and the reversal times.The K562/A02 cells treated with CsA,and reverse transcriptase(RT)-PCR technique was used to examine the mdr1 and H2AX mRNA level.Flow cytometry was used to measure P-glycoprotein(P-gp) and H2AX expression.Neutral comet assay was used to detect the level of DNA double strands break(DSBs) of the K562/A02 treated with ?H2AX antibody.Results 2?10-3g/L CsA could increase the sensitivity of K562/A02 to ADM,and the reversal times was 5.28.Mdr1 mRNA level and H2AX mRNA level were decreased when treated with CsA(P
