Expression of Hypoxia Inducible Factor-1? in Human Renal Proximal Tubular Cells Injuried by Hypoxia/Reoxygenation and the Intervention Effect of Danshen
- VernacularTitle:缺氧诱导因子-1?在新生儿窒息后缺氧/复氧肾脏人近曲肾小管上皮细胞损伤中的表达及丹参干预效果
- Author:
lin, GUO
;
wen-bin, DONG
;
ming-yong, WANG
;
cun-liang, DENG
- Publication Type:Journal Article
- Keywords:
hypoxia inducible factor-1?;
Danshen;
human renal proximal tubular cells;
hypoxia/reoxygenation
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore expression of hypoxia inducible factor-1?(HIF-1?)in kidney cells during hypoxia/reoxygenation injury,and to study the effect of Danshen on prevention of the hypoxia/reoxygenation injury to cells.Methods Human renal proximal tubular cells(HK-2)were used as the target cell.There were 3 groups:control group,model group,Danshen group.hypoxia/reoxygenation models after neonatal asphyxia were established with liquid paraffin covering method.Expression of HIF-1? were detected with strcp avidin biotin complex(SABC)immunohistochemistry at following different time points:hypoxia 1,4,8,12,24 h,which means 1,4,8,12,24 h respectively after hypoxia;and hypoxia/reoxygenation 1,4,8,12,24 h,which means 1,4,8,12,24 h respectively after hypoxia/reoxygenation.Results HIF-1? was expressed mainly in HK-2's nucleus.There had low expression of HIF-1? in HK-2 cells under the normal culture,and its expression level kept rising quickly during hypoxic/reoxygenatic culture,until 4 h after the beginning of reoxygenation.Compared with the study group,the expression level of HIF-1? in HK-2 cells of the Danshen group were significantly lower at different time points(Pa
