Effect of Hypoxia-Ischemia on Expression of Second Mitochondria-Derived Activator of Caspase Protein in Neonatal Rats
- VernacularTitle:缺氧缺血对新生大鼠脑组织半胱氨酸蛋白水解酶激活因子蛋白表达的影响
- Author:
yong-xiang, CHEN
;
guo-yuan, WU
- Publication Type:Journal Article
- Keywords:
hypoxia;
rat,newborn;
brain;
second mitochondria-derived actirator of caspase protein
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hypoxia-ischemia(HI)on expression of second mitochondria-derived activator of caspase proteinc(Smac)in brain tissue of neonatal rats.Methods All neonatal rats were divided randomly into HI group,pseudo-operation group,and normal control group.The animal models of HIE were made.At 24,48,72,96 h after HI,Western blot was used to detect the expression of Smac protein in hippocampus.The relative activity of Caspase-3 in hippocampus tissue was determined by colorimetric assay.Results In HI group,at 24,48,72,96 h after HI,the expression levels of Smac protein in hippocampus significantly increased compared with that of pseudo-operation group and normal control group(Pa
