Establishment of Animal Model for Study of Children with Moderate Blood Lead Levels with Young Rabbits
- VernacularTitle:儿童慢性中度铅中毒幼兔动物模型的建立
- Author:
gao-yan, YU
;
chong-huai, YAN
;
xiao-gang, YU
;
yong, ZUO
;
xiang-yu, ZOU
;
sheng-hu, WU
;
jian, XU
;
yan-ping, ZHANG
- Publication Type:Journal Article
- Keywords:
lead;
animal model;
juvenile stage;
rabbit
- From:Journal of Applied Clinical Pediatrics
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the animal model for the study of children with moderate blood lead levels in young rabbits,for the study of the ideal therapy for moderate lead poisoning in children.Methods Sixteen 45-day-old male New Zealand rabbits were randomly divided into control and lead-exposed group,8 in each group.Rabbits in the lead-exposed group were treated with 5 mg/(kg?d)lead acetate in their forage for 6 weeks to establish moderate lead poisoning animal model.The blood lead levels(BLLs)were determined by atomic absorption spectrometer(AAS),and the urine lead levels and the lead concentrations of tissue and organ were determined by inductively coupled plasma-mass spectrometry(ICP-MS).Histopathology in tissue and organ was observed under the light microscope.Results The BLLs and the urine lead levels in lead-exposed group step up rapidly in primal weeks,then retained at a steady levels.The BLLs exhibited moderate level BLLs during the lead exposure period.Compared with control group,the body weight gain,testis and hippocampus wet coefficient of the lead-exposed group significantly decreased(P_a
