Expression and Significance of Matrix Metalloproteinase and Tissue Inhibitor of Metalloproteinase-1 in Rats with Glomerular Sclerosis
- VernacularTitle:基质金属蛋白酶及金属蛋白酶组织抑制因子-1在肾小球硬化大鼠中的表达和意义
- Author:
feng-ying, LEI
;
yuan-han, QIN
;
juan, PEI
;
peng, HU
- Publication Type:Journal Article
- Keywords:
matrix metalloproteinase;
tissue inhibitor of metalloproteinase;
extracellular matrix;
glomerular sclerosis
- From:Journal of Applied Clinical Pediatrics
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expression and significance of matrix metalloproteinase-2(MMP-2),MMP-9 and tissue inhibitor of metalloproteinase-1(TIMP-1) in rats with glomerular sclerosis made by doxorubicin.Methods Forty Wistar male rats(8-week-old) were randomly assigned into 2 groups:sham operated and model groups.Rats in model group were nephrectomized after anesthesia and injected with adriamycin(5 mg/kg) after 1 week.Rats in sham operated group was subjected to sham operation and injected with normal saline after 1 week through the tail vein.All rats were killed in the 12th week.Immuno-histochemistry was performed on renal tissue to detect Collagen Ⅳ(Col-Ⅳ),fibronectin(FN),MMP-2,-9 and TIMP-1.Results Immunohistochemistry staining indicated that expressions of MMP-2,-9 in model group decreased significantly compared to sham operated group(Pa
