Comparison of agar-gel diffusion tests, counterimmunoelectrophoresis and enzyme-linked immunosorbent assay in the sera of skin test positives for paragonimiasis.
10.3347/kjp.1983.21.2.270
- Author:
Ok Ran LEE
1
;
Won Young CHOI
Author Information
1. Department of Parasitology, College of Medicine, Soonchunhyang University, Choong-Nam, Korea.
- Publication Type:Original Article
- MeSH:
parasitology-helminth-trematoda;
Clonorchis sinensis;
clonorchiasis;
ELISA;
immunology;
diagnosis;
paragonimiasis;
Paragonimus westermani;
agar-gel diffusion;
counterimmunoelectrophoresis
- From:The Korean Journal of Parasitology
1983;21(2):270-280
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Agar-gel diffusion test (AGD), counterimmunoelectrophoresis (CIEP) and enzyme-linked immunosorbent assay(ELISA) were examined with the sera of skin test positives for paragonimiasis. The crude antigen(Paragonimus whole worm extracts: protein concentration, 7.56mg/ml) and human sera were used in AGD and CIEP. And in ELISA test, diluted antigen with 1:40,000 of crude antigen and diluted sera with 1:100, 1:200 were used in the test. The positive identical ratio between AGD and CIEP reactions is 98 percent and negative identical ratio is 100 percent. One or three precipitin bands are observed in AGD. One to seven precipitin bands are also revealed in CIEP. Especially, deeply stained bands are observed in CIEP than those of AGD. The positive identical ratios between AGD and ELISA tests are 96 percent in 1:100 diluted sera, and 94 percent in 1:200 diluted sera. But the negative identical ratios between AGD and ELISA tests are 97 percent and 99 percent respectively in 1:100 and 1:200 diluted sera. The positive identical ratios between CIEP and ELISA tests are 98 percent and 96 percent respectively in 1:100 and 1:200 diluted sera, but also 97 percent and 99 percent in 1:100 and 1:200. Control sera, such as clonorchiasis, amoebiasis and toxoplasmosis, revealed all negatives with Paragonimus antigen in AGD, CIEP and ELISA tests. By above results, ELISA was most sensitive, next CIEP and AGD. But AGD test appears to be more useful when used to crude antigen without cross reaction with other parasitic infections. CIEP test is basically equal in terms of precipitin reaction, but CIEP is able to be detected more sensitively and rapidly though less simple in handiwork than AGD. Consequently, three methods for immunological tests of paragonimiasis have good correlations with one another. Also, each of these has both merits and demerits in immunological test for paragonimiasis. But the ELISA test was proved to be the most sensitive and convenient tool for mass screening test, especially in case of using purified antigen.
