Expressions of Surfactant Protein A,B and Aquaporin-5 mRNA in Premature Rats with Hyperoxia-Induced Chronic Lung Disease and Those Significances
- VernacularTitle:高氧致慢性肺疾病早产鼠肺组织水通道蛋白-5及表面活性蛋白-A、B基因表达的意义
- Author:
jian-hua, FU
;
feng-ying, YU
;
li, PAN
;
xin-dong, XUE
- Publication Type:Journal Article
- Keywords:
surfactant protein;
aquaporin;
alveolar epithelial cell;
differentiation;
hyperoxia
- From:Journal of Applied Clinical Pediatrics
2006;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the dynamic changes of expressions of surfactant protein A,B(SP-A,B)and aquaporin5(AQP5)mRNA of specificity mark in alveolar epithelial cell(AEC)of premature rats with chronic lung disease(CLD)and those significances.Methods Eighty premature rats were randomly and equally divided into model group(hyperoxia group)and control group(room air group).CLD was induced by hyperoxia exposure.The gene expressions of SP-A,SP-B and AQP5 were assayed with reverse transcription polymerase chain reaction(RT-PCR)after 1,3,7,14 and 21 days.Results Compared with control group,in hyperoxia group,the levels of SP-A and SP-B mRNA increased from day 7(Pa
