Effect of Shenfu Injection on Neuron Apoptosis Caused by Hypoxic-Ischemic Brain Damage in Neonatal Rats′ Hippocampus
- VernacularTitle:参附注射液对缺氧缺血性脑损伤新生大鼠海马神经元凋亡影响
- Author:
jun, WANG
;
li-juan, YANG
;
yan-fang, HE
;
qun-e, CHEN
;
shao-mei, ZHANG
- Publication Type:Journal Article
- Keywords:
shenfu injection;
hypoxic-ischemic encephalopathy;
Bcl-2;
Bax;
apoptosis;
hippocampa
- From:Journal of Applied Clinical Pediatrics
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of shenfu injection on the neuron apoptosis in hippocampal CA1 region of newborn with hypoxic-ischemic brain damage(HIBD).Methods The experiment included 2 parts.One was to measure the apoptosis rate by the flow cytometry,and the other was to investigate the expression of Bcl-2 and Bax of neurons in left hippocampal CA1 region.Models of postnatal 7-day Sprague-Dawley(SD)rats with HIBD were established,and were equally divided into 4 groups:sham operation(group S),control group(group C),shenfu injection pretreatment(group P),and shenfu injection treatment(group SF).The neuron apoptosis rate in hippocampal CA1 region in every group was measured at 2 h before and 2,12,24 h,3,7,14 and 28 d after hypoxic ischemic(HI) insult.The expressions of Bcl-2 and Bax were performed by immunohisochemistry.Results The apoptosis of neuron in hippocampal CA1 region in group P and group SF after HI insult was significantly less than that of group C(P
