Expression of GATA-3 in Pulmonary Tissue of Asthmatic Mice and Inhibitory Effect of Dexamethsone on It
- VernacularTitle:GATA-3在支气管哮喘小鼠肺组织表达及地塞米松对其抑制作用
- Author:
hai-guo, YU
;
xiao-qing, QIAN
;
li-juan, QIAN
- Publication Type:Journal Article
- Keywords:
GATA-3;
asthma;
dexamethsone;
mouse
- From:Journal of Applied Clinical Pediatrics
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of GATA-3 in pulmonary tissue of asthmatic mice and the inhibitory effect of dexamethsone(Dex)on it.Methods The Blab/c mice asthma model was induced by ovalbumin(OVA) with classic method.Twenty-four male mice were randomly divided into control group,asthmatic group and Dex treated group.The expression of GATA-3 protein was measured by immunohistochemical staining.The expression of GATA-3 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).The level of IL-4 in mice spleen CD4 T cell was measured by flow cytometry.The airway inflammation was evaluated by HE staining.Results The percentages of postive GATA-3,GATA-3 mRNA and IL-4 protein of asthmatic group were significantly higher than those of control group(P
